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KMID : 1234420100380020144
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Korean Journal of Microbiololgy and Biotechnology
2010 Volume.38 No. 2 p.144 ~ p.150
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Isolation and Characteristics of Alginate-Degrading Methylobacterium sp. HJM27
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Kim Ok-Ju
Lee Dong-Geun
Lee Sung-Mok
Lee Suck-June
Do Hyung-Joo
Park Hye-Jin
Kim Andre
Lee Jae-Hwa
Ha Jong-Myung
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Abstract
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This study was aimed to screen bacteria of high alginate-degrading activity, to select the nitrogen source and concentration of NaCl and sodium alginate for the production of alginate-degrading enzyme, and to determine reaction conditions of enzyme. A novel alginate-degrading bacterium was isolated from abalone (Haliotis discus hannai) and named Methylobacterium sp. HJM27 by 16S rDNA sequence analysis. The optimum culture conditions for the production of alginate-degrading enzyme were 1.0% sodium alginate, 0.5% peptone, 0.3% yeast extract, 1.5% NaCl, 25oC and 48 hours incubation time. The raw enzyme showed the highest activity at 25oC and pH 9, and produced 1.217 g - reducing sugar per liter in 0.8% (w/v) sodium alginate for 30 minutes. Methylobacterium sp. HJM27 and its alginate-degrading enzyme would be useful for the production of bioenergy and biofunctional oligosaccharides from seaweed.
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KEYWORD
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Alginate, alginate-degrading enzyme, brown algae, Methylobacterium sp., marine organism, seaweed
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